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RGASP - Summary of Evaluation Results

 

On this page you can find the links to the RGASP(external link) assessment results as well as the graphical analysis of those results.

An updated version of the RGASP analyses has been published on Nature Methods journal, along with an assessment of RNA-seq mapping tools, here you can find the reference to full text:
Assessment of transcript reconstruction methods for RNA-seq




IMPORTANT
The same terms from RGASP project apply to all data on this web site (see further details at Sanger RGASP web pages(external link)).
Data not to be used for publications without written permission (see further details at ENCODE web site(external link)).

 

EVALUATION PROTOCOL

 

Overview of Sensitivity and Specificity Scores

 

Image

 
The accuracy measures being used along this section are described in Burset and Guigó (Genomics, 34/3:353-357, 1996(external link)), Reese et al (Genome Research, 10/4:483-501, 2000(external link)) and Guigó et al (Genome Research, 10/10:1631-1642, 2000(external link)). Figure above was adapted from the first reference (Burset and Guigó, 1996(external link)). Evaluation at gene and transcript level for the results displayed on this site was performed following the EGASP "IMIM" criteria, which is described on the supplementary web materials(external link) for Guigó et al (Genome Biology, 7/Suppl1:S2, 2006(external link)).

Computational Protocol and Software

 
Go to the ''analysis protocol and software'' page

RGASP: ROUND 1

 

Codes for Participants

 

aleAlessandro GuffantilioLior Pachter
artArthur Moisdon marMario Stanke
benBen Brown gunGunnar Raetsch
carCarrie A Davis seaSean Grimmond
chrChristian Iseli simSimon White
eliElizabeth Purdom thoThomas Wu
gauGautier Koscielny tylTyler Alioto
gerGerstein lab vicVictor Solovyev
hugHugues Richard xiaXiaowo Wang
jelJeltje van Baren yarYarden Katz
jieJie Wu zefZefeng Zhang
   wolWold/Mortazavi

 

Evaluation Scores Data

 

  HUMAN WORM FLY     Cols Description
Validation Summary tbl tbl tbl tbl
Nucleotide Level Evaluation tbl tbl tbl tbl
Exon Level Evaluation tbl tbl tbl tbl
Gene Level Evaluation (xSEQs) tbl tbl tbl tbl
Gene Level Evaluation (xGENEs) tbl tbl tbl tbl

 

Hub of Graphical Results

 

  HUMAN WORM FLY     DESCRIPTION
SNSP
SCATTERPLOTs
hub hub hub SN versus SP for all the submissions on all the sequences.
BOXPLOTs
by SUBMISSION
hub hub hub SNSP at sequence level against submissions for each evaluation variable.
BOXPLOTs
by SEQUENCE
hub hub hub SNSP at submission level against sequences for each evaluation variable.
BOXPLOTs
by VARIABLE
hub hub hub SNSP at sequence level against evaluation variables for each submission.

 
NOTES:

  • The submissions were split into four categories: partial, CDS-only, exon-only and full-predictions. The pages linked from the table summarize, for every evaluation variable taken into consideration, a plot with all submissions together (except for the last row boxplots), followed by four plots, one per each of the mentioned categories. When data is not available for a given variable, an asterisk will mark the corresponding submission, variable or sequence boxplot. When there was no data available for all the elements within a given category on a given variable, an empty plot is shown as placeholder. In example, exon-only predictions do not contain CDS features, thus you get an empty plot for CDS related variables on that category.
  • All scatterplots show data on every submission and every sequence. Each dot corresponds to the SN vs SP on a given sequence for a given submission, no labels though. To check those details, you can look at the box-plots.
  • All boxplots from the above table show (SN+SP)/2 on the y-axis. Box upper and bottom edges correspond to the 3rd and 1st quartiles, the line in the middle is the median. The ticks above and below define the range of two times the interquartile range, which means that points out of those marks can be considered as outlayers. The points marked as outlayers only show their label when they refer to sequences, as the names for the submission files or evaluation variables are too long to display on those plots

 

RGASP: ROUND 2

 

Codes for Participants

 

chrChristian Iseli marMario Stanke
gerGerstein lab seaSean Grimmond
gunGunnar Raetsch simSimon White
hugHugues Richard thoThomas Wu
jieJie Wu tylTyler Alioto
lioLior Pachter vicVictor Solovyev
   wolWold/Mortazavi

 

Annotation Datasets Feature Summaries

 

FLY
SET #CDS #EXON TOTAL FEATS #TRANSCRIPTS
FILT 103107 116534 219641 22584
LOW 9307 10419 19726 2283
MED 44101 49139 93240 9589
HIGH 49696 56973 106669 10712

 

WORM
SET #CDS #EXON TOTAL FEATS #TRANSCRIPTS
FILT 148869 148869 446607 22576
LOW 34036 34036 102108 6090
MED 64545 64545 193635 8140
HIGH 45492 45492 136476 7331

 

HUMAN
SET #CDS #EXON TOTAL FEATS #TRANSCRIPTS
FILT 1048919 795799 1781809 94576
LOW 369822 291578 654698 35024
MED 523206 393843 876955 45013
HIGH 155891 110378 250156 14539

 

  • Feature totals can include other annotation features than cds/exons, like introns.

 

Evaluation Scores Data

 

  HUMAN WORM FLY     Cols Description
Validation Summary tbl %NAY% tbl %NAY% tbl %NAY% tbl
Nucleotide Level Evaluation ALL FIL LOW MED HIG ALL FIL LOW MED HIG ALL FIL LOW MED HIG tbl
Exon Level Evaluation ALL FIL LOW MED HIG ALL FIL LOW MED HIG ALL FIL LOW MED HIG tbl
Gene/Transcript Level Evaluation (xSEQs) ALL FIL LOW MED HIG ALL FIL LOW MED HIG ALL FIL LOW MED HIG tbl
Gene/Transcript Level Evaluation (xGENEs) ALL FIL LOW MED HIG ALL FIL LOW MED HIG ALL FIL LOW MED HIG tbl

 

  • %NAY% means Not Available Yet.

 

Hub of Graphical Results

 

  HUMAN WORM FLY     DESCRIPTION
SNSP
SCATTERPLOTs
hub hub hub SN versus SP for all the submissions on all the sequences.
BOXPLOTs
by SUBMISSION
hub hub hub SNSP at sequence level against submissions for each evaluation variable.
BOXPLOTs
by SEQUENCE
hub hub hub SNSP at submission level against sequences for each evaluation variable.
BOXPLOTs
by VARIABLE
hub hub hub SNSP at sequence level against evaluation variables for each submission.
BOXPLOTs
by ANNOTATION SET
hub hub hub SN comparison among the Filtered, Low, Med and High sets, for every variable on all submission.

 
NOTES: Same points from the previous NOTES on the section about RGASP round 1 apply to the results linked from this table.

 

TO DO

 

  • To add validation summary files.

 


Page last modified on Friday 13 of February, 2015 15:33:58 CET